In Class 12 Boards there will be Case studies and Passage Based Questions will be asked, So practice these types of questions. Study Rate is always there to help you. Free PDF Download of CBSE Class 12 Biology Chapter 11 Biotechnology: Principles and Processes Case Study and Passage Based Questions with Answers Prepared Based on Latest Exam Pattern. Students can solve NCERT Class 12 Biology Case Study Questions Biotechnology: Principles and Processes to know their preparation level.
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In CBSE Class 12 Biology Paper, There will be a few questions based on case studies and passage-based as well. In that, a paragraph will be given, and then the MCQ questions based on it will be asked.
Biotechnology: Principles and Processes Case Study Questions With Answers
Here, we have provided case-based/passage-based questions for Class 12 Biology Chapter 11 Biotechnology: Principles and Processes
Case Study/Passage-Based Questions
Case Study 1: Rama lives in a society where a robbery occurred last night. Robbers came into the flat and murdered the old lady residing there. Police came and restricted entry into the flat. They took samples from the room, where the dead body was found. While examining, they found that there is some blood and tissue in the nails of an old lady. According to their observation, police filtered out their inspection to three suspects viz. servant, cook, and milkman. Finally, after two days of robbery, the police caught the criminal. It was the old lady’s cook. Rama was amazed to see how quickly the police completed and shut the case. She asked the inspector how they did it. The policeman told her that it become possible due to the sample collected from the victim, which lead them to the criminal. The sample taken from nail scraping was amplified using PCR and then tested.
What technique was used by the police to identify the criminal?
(a) DNA fingerprinting (b) Gel electrophoresis
(c) Molecular diagnosis (d) Cloning
Answer: (a) DNA fingerprinting
In PCR, the temperature used to denature the DNA is about
(a) 76°C (b) 25°C
(c) 95°C (d) 40°C
Answer: (c) 95°C
Which of the following statements regarding PCR is correct?
(a) Taq polymerase, which is isolated from the bacterium Thermus aquaticus is stable at low temperature only.
(b) With the help of D N A ligase, t h e complementary sticky ends of the DNA are joined to produce an rDNA.
(c) Since the sequence of primers is complementary to the 5′ end of the template DNA, they anneal to it.
(d) DNA purified from the cell is precipitated by adding hot ethanol.
Answer: (b) With the help of D N A ligase, t h e complementary sticky ends of the DNA are joined to produce an rDNA.
Taq polymerase synthesizes DNA region between the primers using
(a) Mg2+ (b) dNTPs
(c) DNA ligase (d) both (a) and (b)
Answer: (d) both (a) and (b)
Given below are the steps of the polymerase chain reaction
Select the option that correctly mentions the sequence in which they occur.
(a) (ii) → (iii) → (i) (b) (i) → (ii) → (iii)
(c) (iii) → (i) → (ii) (d) (ii) → (i) → (iii)
Answer: (a) (ii) → (iii) → (i)
Case Study/Passage-Based Questions
Case Study 2: The DNA, which is transferred from one organism into another by joining it with the vehicle DNA is called passenger or foreign DNA. Generally, three types of passenger DNAs are used. These are complementary DNA (cDNA), synthetic DNA (cDNA), and random DNA. Complementary DNA (cDNA) is synthesized on an RNA template (usually mRNA) with the help of reverse transcriptase. Synthetic DNA (cDNA) is synthesized on a DNA template or without a template. Random DNA is small fragments formed by breaking a chromosome of an organism in the presence of restriction endonucleases.
Reverse transcriptase enzyme was discovered by
(a) Temin and Baltimore
(b) Cohen and Boyer
(c) Arber and Nathan (d) Paul Berg.
Answer: (a) Temin and Baltimore
During cDNA formation, what would happen if DNA formed by reverse transcriptase is not treated with the alkali?
(a) cDNA will not be digested
(b) mRNA will not be digested
(c) Hydrogen bonds will not form between base pairs
(d) mRNA will not be formed
Answer: (b) mRNA will not be digested
The enzyme that helps in the formation of double stranded cDNA is
(a) DNA synthetase (b) ligase
(c) DNA polymerase (d) helicase
Answer: (c) DNA polymerase
DNA polymerase can be obtained form
(a) retrovirus (b) Agrobacterium
(c) tobacco mosaic virus
(d) Thermus aquaticus.
Answer: (d) Thermus aquaticus.
DNA synthesized without a template is referred to as
(a) complementary DNA
(b) random DNA
(c) synthetic DNA (d) Z-DNA.
Answer: (c) synthetic DNA
Case Study 3: Gene manipulation is a fast-emerging science. It started with the development of recombinant DNA molecules. It is named variously as DNA manipulation biotechnology, recombinant DNA technology, and genetic engineering. This technology, which mostly involves cutting and pasting of desired DNA fragments, is based on two important discoveries in bacteria, i.e., the presence of plasmid in bacteria and restriction endonucleases. Paul Berg was able to introduce a gene of SV-40 into a bacterium. The science of recombinant DNA technology took birth when Cohen and Boyer (1973) were able to introduce a piece of gene containing foreign DNA into the plasmid of E.coli.
(i) Biotechnology is also known as
| (a) DNA manipulation biotechnology | (b) recombinant DNA technology |
| (c) genetic engineering | (d) all of these. |
Answer: (d) all of these.
(ii) A bacterial plasmid is a/an
| (a) extra chromosomal material that do not replicate | (b) extra chromosomal material that undergo replication with or without chromosomal DNA |
| (c) tubular structures that help in conjugation | (d) bristle like solid structure that help in adhesion. |
Answer: (b) extra chromosomal material that undergo replication with or without chromosomal DNA
(iii) Father of genetic engineering is
| (a) Paul Berg | (b) Arber | (c) Nathan | (d) Smith. |
Answer: (a) Paul Berg
(iv) Which of the following is used by Paul Berg to introduce a gene of SV-40 in a bacterium?
| (a) E. coli | (b) cos-plasmids | (c) Lambda phage | (d) None of these |
Answer: (c) Lambda phage
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